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1.2

Minor Versionm

by Procheta Mallik

    • Be careful with the boiling water, stay away as the leaf boils.

    • Handle scissors/cutter with care.

    • Make sure to wash your hands after handling the cocopeat/mud.

  1. Cut the bottle into 2 pieces about two-thirds the way up the bottle with the help of the cutter/scissor Repeat this with another bottle. It's okay if both the bottles vary in size.
    • Cut the bottle into 2 pieces about two-thirds the way up the bottle with the help of the cutter/scissor

    • Repeat this with another bottle.

    • It's okay if both the bottles vary in size.

  2. Mark three points on the bottle cap, and with the help of a pair of scissors, pierce through these points making holes. Insert a piece of cotton cloth into each hole. Repeat the same with the cap of the bottle #2.
    • Mark three points on the bottle cap, and with the help of a pair of scissors, pierce through these points making holes.

    • Insert a piece of cotton cloth into each hole.

    • Repeat the same with the cap of the bottle #2.

    • It's okay if the holes are not of the exact same diameter, as long as they snugly fit the cotton cloths.

  3. Pull the cloth through the holes a little bit, and screw the cap on as shown. Do this for bottle #2 as well. Invert the top part of the bottle, and place it on top of the bottom part (look at the picture for reference). Now pour the cocopeat in the top part of the bottle, so that around half of it is full.
    • Pull the cloth through the holes a little bit, and screw the cap on as shown. Do this for bottle #2 as well.

    • Invert the top part of the bottle, and place it on top of the bottom part (look at the picture for reference).

    • Now pour the cocopeat in the top part of the bottle, so that around half of it is full.

    • Lift the inverted top part and add water into the bottom bottle, until the ends of the cloth pieces are completely immersed.

    • In each of the bottles, plant 4 -5 bean seeds in the soil/cocopeat, and pat down the soil.

  4. Grow both the bean plants for 2 weeks, until the plants have grown enough as shown in the picture.
    • Grow both the bean plants for 2 weeks, until the plants have grown enough as shown in the picture.

    • The plants might not be of the exact same size - we only need a couple of leaves to grow fully.

    • Now, place one plant in a dark room with no trace of sunlight for the next three days.

    • And the other one in the presence of sunlight (by the window, or outside) for those three days.

    • If you can't find a dark place to place the plant in, place a dark (black) garbage bag over the plant, completely shielding it from sunlight.

  5. After the three days, pluck one leaf from each of the plants, and keep track of which one was in the sun. Now start the experiment with one leaf: boil it in hot water, kept in a vessel on a lit burner, for around 2 minutes. Don't write on the leaf to label as the boiling water will wash it away.
    • After the three days, pluck one leaf from each of the plants, and keep track of which one was in the sun.

    • Now start the experiment with one leaf: boil it in hot water, kept in a vessel on a lit burner, for around 2 minutes.

    • Don't write on the leaf to label as the boiling water will wash it away.

    • The leaves may or may not show physical differences, do not worry either ways.

    • You can start the experiment with either leaf first.

  6. After the 2 minutes, place leaf #1 in a test tube containing propanol (or any alcohol/spirit) Place the test tube in the vessel containing boiling water - a water bath - until the leaf discolours. Fill the alcohol until about half the height of the tube.
    • After the 2 minutes, place leaf #1 in a test tube containing propanol (or any alcohol/spirit)

    • Place the test tube in the vessel containing boiling water - a water bath - until the leaf discolours.

    • Fill the alcohol until about half the height of the tube.

    • The alcohol will start to boil (bubble and rise to the top), this is when the leaf will start losing its colour.

  7. Remove the test tube from the hot water, and turn off the burner. Remove the leaf from the tube, and place it on a white tile/sheet. And add a few drops of Iodine solution to it and watch what happens.
    • Remove the test tube from the hot water, and turn off the burner.

    • Remove the leaf from the tube, and place it on a white tile/sheet.

    • And add a few drops of Iodine solution to it and watch what happens.

    • Repeat steps 7 to 10 with leaf #2! Compare your results.

    • If the cotton cloth pieces are not submerged in water because they are not long enough, just cut the bottle to make it shorter or use new cotton pieces.

    • Make sure to plant an adequate number of seeds (4-6) so that at least a couple of them germinate.

    • If there isn't enough cocopeat / soil to fill half the bottle, just add more of it.

    • If the cloth isn't absorbent, find one that is!

    • Keep track of which leaf was in the sun and which wasn't; don't interchange them

    • Did both the plants grow similarly in the first two weeks?

    • Did you 'see' any changes after separating the plants for those three days?

    • After boiling the leaves, and performing the Iodine test on both, what did you see?

    • Did both the leaves turn dark blue (indicating the presence of starch)? If not, why?

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