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Minor Versionm

by Procheta Mallik

    • Be careful with the boiling water, stay away as the leaf boils.

    • Handle scissors/cutter with care.

    • Make sure to wash your hands after handling the cocopeat/mud.

  1. Cut the bottle into 2 pieces about two-thirds the way up the bottle with the help of the cutter/scissor
    • Cut the bottle into 2 pieces about two-thirds the way up the bottle with the help of the cutter/scissor

    • Repeat this with another bottle.

    • It's okay if both the bottles vary in size.

    • Mark three points on the bottle cap, and with the help of a pair of scissors, pierce through these points making holes.

    • Insert a piece of cotton cloth into each hole.

    • Repeat the same with the cap of the bottle #2.

    • It's okay if the holes are not of the exact same diameter, as long as they snugly fit the cotton cloths.

    • Pull the cloth through the holes a little bit, and screw the cap on as shown. Do this for bottle #2 as well.

    • Invert the top part of the bottle, and place it on top of the bottom part (look at the picture for reference).

    • Now pour the cocopeat in the top part of the bottle, so that around half of it is full.

    • Lift the inverted top part and add water into the bottom bottle, until the ends of the cloth pieces are completely immersed.

    • In each of the bottles, plant 4 -5 bean seeds in the soil/cocopeat, and pat down the soil.

    • Grow both the bean plants for 2 weeks, until the plants have grown enough as shown in the picture.

    • The plants might not be of the exact same size - we only need a couple of leaves to grow fully.

    • Now, place one plant in a dark room with no trace of sunlight for the next three days.

    • And the other one in the presence of sunlight (by the window, or outside) for those three days.

    • If you can't find a dark place to place the plant in, place a dark (black) garbage bag over the plant, completely shielding it from sunlight.

    • After the three days, pluck one leaf from each of the plants, and keep track of which one was in the sun.

    • Now start the experiment with one leaf: boil it in hot water, kept in a vessel on a lit burner, for around 2 minutes.

    • Don't write on the leaf to label as the boiling water will wash it away.

    • The leaves may or may not show physical differences, do not worry either ways.

    • You can start the experiment with either leaf first.

    • After the 2 minutes, place leaf #1 in a test tube containing propanol (or any alcohol/spirit)

    • Place the test tube in the vessel containing boiling water - a water bath - until the leaf discolours.

    • Fill the alcohol until about half the height of the tube.

    • The alcohol will start to boil (bubble and rise to the top), this is when the leaf will start losing its colour.

    • Remove the test tube from the hot water, and turn off the burner.

    • Remove the leaf from the tube, and place it on a white tile/sheet.

    • And add a few drops of Iodine solution to it and watch what happens.

    • Repeat steps 7 to 10 with leaf #2! Compare your results.

    • If the cotton cloth pieces are not submerged in water because they are not long enough, just cut the bottle to make it shorter or use new cotton pieces.

    • Make sure to plant an adequate number of seeds (4-6) so that at least a couple of them germinate.

    • If there isn't enough cocopeat / soil to fill half the bottle, just add more of it.

    • If the cloth isn't absorbent, find one that is!

    • Keep track of which leaf was in the sun and which wasn't; don't interchange them

    • Did both the plants grow similarly in the first two weeks?

    • Did you 'see' any changes after separating the plants for those three days?

    • After boiling the leaves, and performing the Iodine test on both, what did you see?

    • Did both the leaves turn dark blue (indicating the presence of starch)? If not, why?

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