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2.2

Major VersionM

by Raghottam Joshi

Introduction

Germination is the process of a seed developing into a new plant and depends on the amount of water, air, temperature and sunlight supplied to it. Water plays an important role for seeds to germinate.

In this TACtivity, we compare the germination rate of different seeds when fully submerged in water, partially submerged in water and exposed to air without water.

Video Overview

    • Water stored for many days may turn contaminated and become a breeding ground for mosquitoes and diseases. Replace with fresh water every few days.

    • Do not consume either the soaked seeds or the water.

    • Be careful while handling the heated binding wire used to make holes.

  1. Collect three different types of seeds (chickpea, green gram and paddy). Soak the seeds in water for 3 - 4 hours. Soak the seeds in water for 3 - 4 hours.
    • Collect three different types of seeds (chickpea, green gram and paddy).

    • Soak the seeds in water for 3 - 4 hours.

  2. After 3 - 4 hours, drain the water and collect the seeds. Observe that the seeds are swollen after 4 hours.
    • After 3 - 4 hours, drain the water and collect the seeds.

    • Observe that the seeds are swollen after 4 hours.

  3. Take a fat straw and mark a line at 15 cm on it, from one end. Cut at the marked line using scissors. Repeat the steps with the other fat straw.
    • Take a fat straw and mark a line at 15 cm on it, from one end.

    • Cut at the marked line using scissors.

    • Repeat the steps with the other fat straw.

  4. Mark lines at 1 cm, 7.5 cm and 14 cm, respectively, on both 15 cm fat straws. Take the frooti straw and draw its circular outline on either sides of all the marked lines as shown in the picture. Take the frooti straw and draw its circular outline on either sides of all the marked lines as shown in the picture.
    • Mark lines at 1 cm, 7.5 cm and 14 cm, respectively, on both 15 cm fat straws.

    • Take the frooti straw and draw its circular outline on either sides of all the marked lines as shown in the picture.

  5. After marking the holes on either sides of the marked lines, heat a thick binding wire on a candle flame. Using the heated thick binding wire, make holes at all the marked circular outlines on the straw. Ensure that the binding wire comes out from the other side, while making holes. Using the heated thick binding wire, make holes at all the marked circular outlines on the straw. Ensure that the binding wire comes out from the other side, while making holes.
    • After marking the holes on either sides of the marked lines, heat a thick binding wire on a candle flame.

    • Using the heated thick binding wire, make holes at all the marked circular outlines on the straw. Ensure that the binding wire comes out from the other side, while making holes.

  6. Follow the same procedure for making holes on the other fat straw, as shown in the first picture. Mark a line at 6 cm on the frooti straw, as shown in the second picture. Cut at the marked line using scissors.
    • Follow the same procedure for making holes on the other fat straw, as shown in the first picture.

    • Mark a line at 6 cm on the frooti straw, as shown in the second picture.

    • Cut at the marked line using scissors.

  7. Similarly, make 6 pieces of frooti straw, each being 6 cm in length. Pass the frooti straws into the holes made on the fat straw, as shown.
    • Similarly, make 6 pieces of frooti straw, each being 6 cm in length.

    • Pass the frooti straws into the holes made on the fat straw, as shown.

  8. Insert the frooti straw pieces into the holes of the fat straws, as shown in the first picture. After inserting all the frooti straw pieces, the straw assembly will resemble the second picture shown here. Gently pull the fat straws away from each other and towards the frooti straw ends, to make it appear like a ladder, as shown in the third picture.
    • Insert the frooti straw pieces into the holes of the fat straws, as shown in the first picture.

    • After inserting all the frooti straw pieces, the straw assembly will resemble the second picture shown here.

    • Gently pull the fat straws away from each other and towards the frooti straw ends, to make it appear like a ladder, as shown in the third picture.

  9. Apply three evenly spaced dots of glue on all the rungs of the ladder (frooti straws). Place the seeds on the glue dots. Do not apply too much glue.
    • Apply three evenly spaced dots of glue on all the rungs of the ladder (frooti straws).

    • Place the seeds on the glue dots.

    • Do not apply too much glue.

  10. Place the seed and ladder setup inside a glass/transparent container. Fill the container with water till it reaches the centre rung of the ladder, such that the seeds on the centre rung are half exposed to air and half immersed in water.
    • Place the seed and ladder setup inside a glass/transparent container.

    • Fill the container with water till it reaches the centre rung of the ladder, such that the seeds on the centre rung are half exposed to air and half immersed in water.

    • Observe the setup everyday.

  11. Day 1 -  Chickpea and green gram seeds sprout in the partially submerged region. Day 2 -  All the three seeds in the partially submerged region show shoot growth. Seed cover of chickpea and green gram seeds in submerged region is ruptured. Day 3 -  Green gram seed in the partially submerged region show root growth. Chickpea and green gram seeds sprout in the submerged region.
    • Day 1 - Chickpea and green gram seeds sprout in the partially submerged region.

    • Day 2 - All the three seeds in the partially submerged region show shoot growth. Seed cover of chickpea and green gram seeds in submerged region is ruptured.

    • Day 3 - Green gram seed in the partially submerged region show root growth. Chickpea and green gram seeds sprout in the submerged region.

  12. Day 4 - Seeds, in the partially submerged region, are growing but no growth is seen in the seeds that are totally exposed to air. Day 5 - Chickpea and green gram seeds in the partially submerged region show leaf bud development. Day 6 - Chickpea and green gram seeds, in the partially submerged region, grow into a seedling with leaves.
    • Day 4 - Seeds, in the partially submerged region, are growing but no growth is seen in the seeds that are totally exposed to air.

    • Day 5 - Chickpea and green gram seeds in the partially submerged region show leaf bud development.

    • Day 6 - Chickpea and green gram seeds, in the partially submerged region, grow into a seedling with leaves.

    • No growth - Make sure you check the water level in the container everyday such that the centre seeds remain partially submerged throughout your experiment (2-6 days or even longer if you want).

    • Seeds are decaying - Change water regularly.

    • Setup starts stinking - Empty the container and fill it with fresh water.

  13. Try growing seeds in different concentration of glucose solution. Try growing seeds in different concentration of salt solution. Try growing seeds in different acidic medium such as vinegar and citric acid.
    • Try growing seeds in different concentration of glucose solution.

    • Try growing seeds in different concentration of salt solution.

    • Try growing seeds in different acidic medium such as vinegar and citric acid.

    • For more variations, Go to Plant Life - Germination (Variations).

Madhushree HS

Member since: 05/02/2017

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