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1.2

Minor Versionm

by Procheta Mallik

    • Exercise caution while handling the glass items.

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  1. Make a clean hole in the cardboard strip slightly less than the bead diameter using scissors.
    • Make a clean hole in the cardboard strip slightly less than the bead diameter using scissors.

    • The hole created in cardboard strip is slightly smaller in size so that bead would fit in there tightly enough.

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    • Make hole in the foam piece of the same size as done in cardboard strip.

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    • Use a non transparent tape and make hole in it of equal size of that in foam piece and stick both of them on cardboard strip.

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    • Place the bead in the hole and push it further inside so that any part the is not protruding out from the object side.

    • Make sure the hands are clean enough while handling the bead otherwise the image would not be clear enough.

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    • Remove the petals to make the removal of pollen convenient from the flower.

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    • Add few drops of water on to the slide and gently dap the front part(with brushes) of the filamnet on water.

    • some powder or dust will fall off the filament and stick to the water.

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    • While putting on cover slip keep the cover slip at some angle with slide initially and lower it gradually to cover the sample.

    • Blot out excess water from sides with some good water absorbent cloth or tissue paper and apply tape on one side to fix the cover slip on slide

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    • Follow the same procedure as in step 1 of flower 1

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    • Take out the filaments with the anthers and remove sticky powder from inside the anthers(colour of this powder is white in most of the flowers).

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    • Follow same procedure as in step 3 of flower 1

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    • Follow same procedure as in step 1 of flower 1

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    • Gently strike the pistil of the flower on dry slide (unlike flower 1) to get yellow dust on the slide

    • Cover the sample with cover slip and apply tape.

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    • Cut a square slot out of cardboard strip and place the glass slide with sample on it to use rubber bands to hold them together

    • Now put back side(side of cardboard other than with foam) of microscope very close(almost touching) on to the sample and watch tiny structures of pollens,.

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    • The micro structure of pollen of different flowers may differ.

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Finish Line

Suhail Ahanger

Member since: 05/02/2017

3,665 Reputation

54 Guides authored

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