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by Raghottam Joshi

Introduction

Observe the pattern of pollen in different flowers using your own DIY Microscope.

  1. DIY Microscope is a prerequisite TACtivity that has to be completed before commencing this TACtivity. In the DIY Microscope, you create your own Microscope with two glass beads of 4.5mm and 2.5mm diameter. You also learn how to focus the microscope. Go to DIY Microscope to complete that TACtivity first.
    • DIY Microscope is a prerequisite TACtivity that has to be completed before commencing this TACtivity. In the DIY Microscope, you create your own Microscope with two glass beads of 4.5mm and 2.5mm diameter. You also learn how to focus the microscope. Go to DIY Microscope to complete that TACtivity first.

    • Exercise caution when using paper cutter/scissor.

  2. Take a Hibiscus flower and pluck out the petals to access the stamen easily. Stamens are male reproductive part of flower. It has anthers attached to a filament. Stamens are male reproductive part of flower. It has anthers attached to a filament.
    • Take a Hibiscus flower and pluck out the petals to access the stamen easily.

    • Stamens are male reproductive part of flower. It has anthers attached to a filament.

  3. Carefully remove the cover from both sides of the slide. Gently tap the anthers onto a slide. A yellow particles fall off the filament like dust. Using wooden skewer, separate the yellow particles to obtain pollen (powder).
    • Carefully remove the cover from both sides of the slide.

    • Gently tap the anthers onto a slide. A yellow particles fall off the filament like dust.

    • Using wooden skewer, separate the yellow particles to obtain pollen (powder).

  4. While placing the cover slip on the slide, keep it at an angle with the slide initially. Then lower it gradually to cover the sample on the slide. Apply clear tape on slide to fix the cover slip on it.
    • While placing the cover slip on the slide, keep it at an angle with the slide initially.

    • Then lower it gradually to cover the sample on the slide.

    • Apply clear tape on slide to fix the cover slip on it.

  5. Observe the sample using  Microscope. View through 4.5 mm bead View through 2.5 mm bead
    • Observe the sample using Microscope.

    • View through 4.5 mm bead

    • View through 2.5 mm bead

  6. Take an other flower (yellow)  and pluck out the petals to easily get the stamen. Choose flowers like May flower to obtain pollen easily. Stamens are male reproductive part of flower. It has anthers attached to a filament.
    • Take an other flower (yellow) and pluck out the petals to easily get the stamen.

    • Choose flowers like May flower to obtain pollen easily.

    • Stamens are male reproductive part of flower. It has anthers attached to a filament.

  7. Remove the cover of slide on both side as shown in step 2. Gently remove anthers from  filament on slide using wooden skewer. Using skewer, separate pollen (powder) from Anthers.
    • Remove the cover of slide on both side as shown in step 2.

    • Gently remove anthers from filament on slide using wooden skewer.

    • Using skewer, separate pollen (powder) from Anthers.

  8. While placing the cover slip on the slide, keep it at an angle with the slide . Then lower it gradually to cover the sample on the slide. Apply tape on slide to fix the cover slip on the it.
    • While placing the cover slip on the slide, keep it at an angle with the slide .

    • Then lower it gradually to cover the sample on the slide.

    • Apply tape on slide to fix the cover slip on the it.

  9. Observe pollen using DIY microscope View through 4.5 mm bead View through 2.5 mm bead
    • Observe pollen using DIY microscope

    • View through 4.5 mm bead

    • View through 2.5 mm bead

    • Not able to get pollen. Choose flowers which has powdery pollen like hibiscus, spider lily, rose, etc.

    • Pollen not visible through microscope. Place it very close to the glass side and view it with one eye closed

    • No clarity of image/ blur image - Check for the stains/impurities on the surface of the microscope bead, glass slide or cover slip

  10. Stain the pollen with safranin and view using Microscope.
    • Stain the pollen with safranin and view using Microscope.

Finish Line

Raghottam Joshi

Member since: 05/02/2017

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