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by Raghottam Joshi

Introduction

These are a set of variations for the TACtivity, Microscope - Fungi.

    • Be careful while handling sharp tools like scissors or paper cutter.

    • Don't touch the fungi mould with bare hands or consume the bread as it may cause itching and rashes.

  1. DIY Microscope is a prerequisite TACtivity that has to be completed before commencing this TACtivity. The DIY Microscope is created with two glass beads of 4.5 mm and 2.5 mm diameter offering a 40 X/100 X magnification. You also learn how to focus the microscope to view the cells of the samples on the slide.
    • DIY Microscope is a prerequisite TACtivity that has to be completed before commencing this TACtivity. The DIY Microscope is created with two glass beads of 4.5 mm and 2.5 mm diameter offering a 40 X/100 X magnification. You also learn how to focus the microscope to view the cells of the samples on the slide.

    • Go to DIY Microscope to complete the TACtivity.

  2. Grow moulds on bread and stain them with methylene blue to reveal structure of yeast.
    • Grow moulds on bread and stain them with methylene blue to reveal structure of yeast.

  3. Take a slice of fresh bread and place it in a plastic cover. Moisten the bread. Do not close the cover air tight. Wait for at least 3-4 days to get a good amount of bread mould.
    • Take a slice of fresh bread and place it in a plastic cover.

    • Moisten the bread. Do not close the cover air tight.

    • Wait for at least 3-4 days to get a good amount of bread mould.

  4. Remove the thin protective plastic covering from both sides of the slide. Wipe the slide with tissue paper.
    • Remove the thin protective plastic covering from both sides of the slide.

    • Wipe the slide with tissue paper.

  5. Add 2-3 drops of methylene blue on the  centre of the slide. Add 2-3 drops of methylene blue on the  centre of the slide.
    • Add 2-3 drops of methylene blue on the centre of the slide.

  6. Using a toothpick, collect a sample of the fungi. With another toothpick spread out the sample as a thin smear. Place the cover slip inclined at an angle on the slide, a few millimetres away from the sample.
    • Using a toothpick, collect a sample of the fungi.

    • With another toothpick spread out the sample as a thin smear.

    • Place the cover slip inclined at an angle on the slide, a few millimetres away from the sample.

    • Slowly, move the top edge of the cover slip towards the sample and cover it fully.

    • Care should be taken to prevent the formation of air bubbles under the cover slip.

  7. Blot around the slide to remove excess stain using tissue paper. Care to be taken to prevent the formation of air bubbles under the cover slip.
    • Blot around the slide to remove excess stain using tissue paper.

    • Care to be taken to prevent the formation of air bubbles under the cover slip.

    • Secure the cover slip with transparent tape so that it does not move while observing.

    • Do not press on the tape as there are chances of it leaving marks.

    • Ensure that your hands are clean when you touch the cover slip.

  8. Place the slide close to the microscope and view it with bright light using an adaptor as shown. Observe the fungi cells using the 4.5 mm bead microscope. Observe the fungi cells using the 4.5 mm bead microscope.
    • Place the slide close to the microscope and view it with bright light using an adaptor as shown.

    • Observe the fungi cells using the 4.5 mm bead microscope.

  9. Place the slide close to the microscope and view it with bright light using an adaptor as shown. Observe the fungi cells using the 2.5 mm bead microscope. Observe the fungi cells using the 2.5 mm bead microscope.
    • Place the slide close to the microscope and view it with bright light using an adaptor as shown.

    • Observe the fungi cells using the 2.5 mm bead microscope.

  10. Make a slide of Trichoderma viride  (plant fungus) in sugar solution with methylene blue stain and observe the structure using DIY Microscope.
    • Make a slide of Trichoderma viride (plant fungus) in sugar solution with methylene blue stain and observe the structure using DIY Microscope.

  11. Take some sugar on a paper. Take a pinch of sugar as shown in the picture. Add the pinch of sugar to the given vial which has Trichoderma viride  in it. Add the pinch of sugar to the given vial which has Trichoderma viride  in it.
    • Take some sugar on a paper. Take a pinch of sugar as shown in the picture.

    • Add the pinch of sugar to the given vial which has Trichoderma viride in it.

  12. Add 1.5 ml of water to the vial using a dropper. Close the lid of the vial and shake the vial well to dissolve the sugar.
    • Add 1.5 ml of water to the vial using a dropper.

    • Close the lid of the vial and shake the vial well to dissolve the sugar.

  13. Remove the thin protective plastic covering from both sides of the slide. Wipe the slide with tissue paper.
    • Remove the thin protective plastic covering from both sides of the slide.

    • Wipe the slide with tissue paper.

  14. Add 1-2 drops of methylene blue on the slide. Add 1-2 drops of methylene blue on the slide.
    • Add 1-2 drops of methylene blue on the slide.

  15. From the vial transfer one drop of the prepared sample to the slide using a dropper. Place the cover slip a few millimetres away from the sample at an angle. Slowly, move the top edge of the cover slip towards the sample and cover it fully.
    • From the vial transfer one drop of the prepared sample to the slide using a dropper.

    • Place the cover slip a few millimetres away from the sample at an angle.

    • Slowly, move the top edge of the cover slip towards the sample and cover it fully.

    • Care should be taken to prevent the formation of air bubbles under the cover slip.

  16. Blot around the slide to remove excess stain using tissue paper. Secure the cover slip with transparent tape.
    • Blot around the slide to remove excess stain using tissue paper.

    • Secure the cover slip with transparent tape.

    • Do not press the tape as there are chances of it leaving marks.

    • Ensure that your hands are clean when you touch the cover slip.

    • Keep the slide undisturbed for one day.

  17. Place the slide close to the microscope and view it with bright light using an adaptor as shown. Observe the Trichoderma structure using the 4.5 mm bead microscope. Observe the Trichoderma structure using the 4.5 mm bead microscope.
    • Place the slide close to the microscope and view it with bright light using an adaptor as shown.

    • Observe the Trichoderma structure using the 4.5 mm bead microscope.

  18. Place the slide close to the microscope and view it with bright light using an adaptor as shown. Observe the Trichoderma structure using the 2.5 mm bead microscope. Observe the Trichoderma structure using the 2.5 mm bead microscope.
    • Place the slide close to the microscope and view it with bright light using an adaptor as shown.

    • Observe the Trichoderma structure using the 2.5 mm bead microscope.

    • Blurred image - Clean the impurities/marks on the surface of the slide or the bead with a soft and clean piece of cloth.

    • Blurred image - Ensure that the microscope is in contact or just above the sample (mounted on the slide).

    • More fungal colonies on slide lead to messy image.

Finish Line

Raghottam Joshi

Member since: 05/02/2017

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