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Minor Versionm

by Suchitha Vishwa

Introduction

Using Benedict's reagent, one can detect the presence of glucose in a food substance. The foods we use are: milk, cornflour solution, cooked dhal, sugar solution and salt solution.

    • Testubes, beaker and Benedict's reagent should be handled carefully.

    • Don't leave the Bunsen Burner unattended when it is on.

    • Please do not ingest any material.

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  1. Take 5 test tubes and add the samples  (around 3 ml) for testing as mentioned below.
    • Take 5 test tubes and add the samples (around 3 ml) for testing as mentioned below.

    • If 5 testubes are not available then do the tests one after the other

    • Test tube A = corn starch solution(1/2 corn starch in 20ml water) , Test tube B = milk,Test tube C = cooked dhal(sambhar)

    • Test tube D = sugar solution(1/2 spoon of sugar in 20ml water) Test tube E = salt solution (1/2 spoon of salt in 20ml water)

    • Take Benedict's reagent in a 50 ml beaker.

    • Add 12-15 drops of Benedict's reagent to each of the 5 test tubes.

    • Observe the color change.

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    • Take a beaker and fill half of it with water.

    • Insert all the 5 test tubes in a beaker and heat it on a Bunsen burner for 10 minutes until the water starts boiling.

    • After water boils for 7-10 minutes put off the burner and set aside the test tubes to cool.

    • Now observe the color change.

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    • Go to the website http://laboratoryinfo.com/benedicts-test... and copy the result interpretation table and compare the results.

    • Starch solution in 'A' is Blue, indicating the absence of reduced sugar.

    • Milk in 'B' is Orange, indicating moderate amount of reduced sugar.

    • Cooked dal in 'C' is Brick Red, indicating large amount of reduced sugar.

    • Sugar solution in 'D' is Green, indicating traces of reduced sugar.

    • Salt Solution in 'E' is Blue, indicating absence of reduced sugar.

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    • Bendict's reagent should be added to samples as mentioned in the steps.

    • Adequate amount of water should be present in the water- bath.

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    • Find the chemicals in Benedict's reagent.

    • This test is conducted to detect the presence of reducing sugars in different foods. What is reducing sugar?

    • Figure out the reason for different colors with different food samples.

    • Try to find out which are the daily foods that contains reduced sugar.

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Suchitha Vishwa

Member since: 05/02/2017

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